Ukrainian Scientific Medical Youth Journal (Sep 2016)

THE «OXIDANTS-ANTIOXIDANTS» SYSTEM IN TUBERCULOSIS/HIV CO-INFECTED PATIENTS WITH PULMONARY TUBERCULOSIS RELAPSES

  • R.M. Yasinskyi

Journal volume & issue
no. 3(96)
pp. 45 – 48

Abstract

Read online

Changes in «oxidants-antioxidants» system play a significant role in the progression of disease in patients with newly diagnosed pulmonary tuberculosis in TB/HIV co-infected patients. The changes in this system haven't been studied yet in tuberculosis/HIV co-infected patients with pulmonary tuberculosis relapses. 31 HIV-positive and 22 HIV-negative patients with pulmonary tuberculosis relapses were examined with the aim to establish the «oxidants-antioxidants» system features in tuberculosis/ HIV co-infected patients with pulmonary tuberculosis relapses. Indicators of protein peroxidation (PP), lipids peroxidation (LP) and glutathione system were measured in patient's blood. Aldehyde-phenylhydrazone, ketone­phenylhydrazone as PP markers were determined in plasma by the method B. Halliwell (1999). Among the lipids peroxidation products malondialdehydes, diene conjugates, triene-ketones and Schiffs bases measured by V.B. Gavrilov method, 1983. The thiol-disulfide system was evaluated by levels: reduced glutathione, glutathione reductase (GR), glutathione peroxidase (GP) and glutathione-transferase (GT) in the cell fraction. Reduced glutathione levels by standard method, (1981), GP, GR by the Beutler E., (1975) method and GT by the method of Habig WH, (1974) were determined.It was found, that there are an increasing of proteins peroxidation markers on the background of the tendency of glutathione reductase and glutathione peroxidase levels reduction in HIV-positive and HIV-negative patients with pulmonary tuberculosis relapses. In HIV-positive patients with relapsed pulmonary tuberculosis there are also increasing levels of lipid peroxidation markers in addition, that due to lower levels of glutathione reductase and glutathione peroxidase worsens the severity of oxidative stress by shifting the balance in the system «oxidant-antioxidant» toward free-radical peroxidation.

Keywords