Isolation and identification of Staphylococcus warneri from diseased Coreius guichenoti

Abstract

Coreius guichenoti is a rare fish found in the upper reaches of the Yangtze River in China and has significant ecological and economic value. In this study, a bacterial strain (F416) was isolated from diseased C. guichenoti and was identified as Staphylococcus warneri. Its median lethal dose (LD50) was 6.31 × 106 colony-forming units/mL. Histopathological observation revealed that substantial inflammatory cell infiltration occurred in the tissues of diseased fish, and the muscle fibers had lesions. Strain F416 is resistant to aminoglycosides, sulfonamides, and tetracyclines used in aquaculture. This is the first study, to the best of our knowledge, to isolate S. warneri from cultured C. guichenoti and to describe its biological characteristics and the histopathological changes that cause disease in C. guichenoti post-infection.

Keywords

• Coreius guichenoti
• Staphylococcus warneri
• Isolation and identification
• Histopathology
• Drug sensitivity