Occurrence and Molecular Characteristics of Microsporidia in Captive Red Pandas (<i>Ailurus fulgens</i>) in China

Jinpeng Yang; Yangyang Zeng; Caiwu Li; Songrui Liu; Wanyu Meng; Wenqing Zhang; Ming He; Liqin Wang; Zhili Zuo; Chanjuan Yue; Desheng Li; Guangneng Peng

doi:10.3390/ani13111864

Animals (Jun 2023)

Occurrence and Molecular Characteristics of Microsporidia in Captive Red Pandas (<i>Ailurus fulgens</i>) in China

Jinpeng Yang,
Yangyang Zeng,
Caiwu Li,
Songrui Liu,
Wanyu Meng,
Wenqing Zhang,
Ming He,
Liqin Wang,
Zhili Zuo,
Chanjuan Yue,
Desheng Li,
Guangneng Peng

Affiliations

Jinpeng Yang: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Yangyang Zeng: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Caiwu Li: China Conservation and Research Center for the Giant Panda, Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, Chengdu 610083, China
Songrui Liu: Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan Academy of Giant Panda, Chengdu 610081, China
Wanyu Meng: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Wenqing Zhang: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Ming He: China Conservation and Research Center for the Giant Panda, Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, Chengdu 610083, China
Liqin Wang: Chengdu Zoo, Chengdu 610051, China
Zhili Zuo: Chengdu Zoo, Chengdu 610051, China
Chanjuan Yue: Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan Academy of Giant Panda, Chengdu 610081, China
Desheng Li: China Conservation and Research Center for the Giant Panda, Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park, Chengdu 610083, China
Guangneng Peng: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

DOI: https://doi.org/10.3390/ani13111864
Journal volume & issue: Vol. 13, no. 11
p. 1864

Abstract

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Enterocytozoon bieneusi and Encephalitozoon spp. are microsporidian pathogens with zoonotic potential that pose significant public health concerns. To ascertain the occurrence and genotypes of E. bieneusi and Encephalitozoon spp., we used nested PCR to amplify the internal transcribed spacer (ITS) gene and DNA sequencing to analyze 198 fecal samples from red pandas from 6 zoos in China. The total rate of microsporidial infection was 15.7% (31/198), with 12.1% (24/198), 1.0% (2/198), 2.0% (4/198) and 1.0% (2/198) for infection rate of E. bieneusi, Encephalitozoon cuniculi, Encephalitozoon intestinalis and Encephalitozoon hellem, respectively. One red panda was detected positive for a mixed infection (E. bieneusi and E. intestinalis). Red pandas living in semi-free conditions are more likely to be infected with microsporidia (χ2 = 6.212, df = 1, p E. bieneusi were found. Three genotypes of E. bieneusi (SC02, D, SCR1) were grouped into group 1 with public health importance, while genotype PL2 formed a separate clade associated with group 2. These findings suggest that red pandas may serve as a host reservoir for zoonotic microsporidia, potentially allowing transmission from red pandas to humans and other animals.

Published in Animals

ISSN: 2076-2615 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine; Science: Zoology
Website: http://www.mdpi.com/journal/animals/

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