Frontiers in Cellular Neuroscience (May 2021)
All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
- Francesca Puppo,
- Sanaz Sadegh,
- Cleber A. Trujillo,
- Cleber A. Trujillo,
- Martin Thunemann,
- Evan P. Campbell,
- Matthieu Vandenberghe,
- Xiwei Shan,
- Xiwei Shan,
- Ibrahim A. Akkouh,
- Evan W. Miller,
- Brenda L. Bloodgood,
- Gabriel A. Silva,
- Anders M. Dale,
- Gaute T. Einevoll,
- Gaute T. Einevoll,
- Srdjan Djurovic,
- Srdjan Djurovic,
- Ole A. Andreassen,
- Alysson R. Muotri,
- Alysson R. Muotri,
- Alysson R. Muotri,
- Alysson R. Muotri,
- Anna Devor,
- Anna Devor
Affiliations
- Francesca Puppo
- Department of Pediatrics/Rady Children’s Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Sanaz Sadegh
- Department of Neurosciences, University of California, San Diego, La Jolla, CA, United States
- Cleber A. Trujillo
- Department of Pediatrics/Rady Children’s Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Cleber A. Trujillo
- Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Martin Thunemann
- Department of Biomedical Engineering, Boston University, Boston, MA, United States
- Evan P. Campbell
- Division of Biological Sciences, Section of Neurobiology, University of California, San Diego, La Jolla, CA, United States
- Matthieu Vandenberghe
- Division of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway
- Xiwei Shan
- Department of Pediatrics/Rady Children’s Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Xiwei Shan
- Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Ibrahim A. Akkouh
- Division of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway
- Evan W. Miller
- Department of Chemistry and Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, United States
- Brenda L. Bloodgood
- Division of Biological Sciences, Section of Neurobiology, University of California, San Diego, La Jolla, CA, United States
- Gabriel A. Silva
- Department of Bioengineering, University of California, San Diego, La Jolla, CA, United States
- Anders M. Dale
- Department of Radiology, University of California, San Diego, La Jolla, CA, United States
- Gaute T. Einevoll
- 0Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway
- Gaute T. Einevoll
- 1Department of Physics, University of Oslo, Oslo, Norway
- Srdjan Djurovic
- 2Department of Medical Genetics, Oslo University Hospital, Oslo, Norway
- Srdjan Djurovic
- 3NORMENT, Department of Clinical Science, University of Bergen, Bergen, Norway
- Ole A. Andreassen
- Division of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway
- Alysson R. Muotri
- Department of Pediatrics/Rady Children’s Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Alysson R. Muotri
- Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA, United States
- Alysson R. Muotri
- 4Kavli Institute for Brain and Mind and Halıcıoglu Data Science Institute, University of California, San Diego, La Jolla, CA, United States
- Alysson R. Muotri
- 5Center for Academic Research and Training in Anthropogeny (CARTA), La Jolla, CA, United States
- Anna Devor
- Department of Biomedical Engineering, Boston University, Boston, MA, United States
- Anna Devor
- 6Department of Radiology, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, United States
- DOI
- https://doi.org/10.3389/fncel.2021.671549
- Journal volume & issue
-
Vol. 15
Abstract
Voltage imaging and “all-optical electrophysiology” in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.
Keywords