Design, synthesis, characterisation and in-vitro antimicrobial activity of some hybridized triazole scaffolds

Abstract

In the present study, twelve hybridized triazole derivatives were synthesized as Glucosamine-6-phosphate synthase inhibitor and evaluated for in-vitro antimicrobial activity. The in-vitro antimicrobial results demonstrated that compound B4b, B4g and B4j possesses potential antibacterial activity against all the tested Gram positive and Gram negative bacterial strains with percentage zone of inhibition 70–89% and antifungal activity against all the screened fungal strains with 85–105%, displaying minimal inhibitory concentration values of 3.125–6.25 μg/mL against bacteria strains and 3.125–12.5 μg/mL against fungi strains compared with standard Gatifloxacin (for bacteria) and Clotrimazole (for fungi). Docking study reviews that the compounds which bind with Ser347, Thr352 and Val399 have significant anti-microbial activity. Comparing antimicrobial activity and docking results, conclude that triazolone derivatives linked through NC with substituted phenyl ring at 4th position seem to be potentially active. The docking study reveals that high affinity of synthesized derivatives (B4b, B4g and B4j) within the binding pocket of glucosamine-6-phosphate synthase strongly enhances the determined activities of these derivatives as potent antimicrobial agents, particularly as antifungal agents.

Keywords

• Glucosamine-6-phosphate synthase
• Triazole
• Antibacterial
• Antifungal