Peptides, solid-phase synthesis and characterization: Tailor-made methodologies

Abstract

Background: Solid-Phase Peptide Synthesis (SPPS) is a mature technique widely used in research and in production. There are different approaches that fulfill the diverse requirements, regarding the number, quantity and quality of peptides. We have implemented three laboratory protocols of synthesis that cover these needs. These protocols have been tested, and the results analyzed with two different sequences used in previous works. Results: The peptide synthesis protocols such as tea bag, microwave synthesis and manual synthesis have allowed obtaining specific yields of 8, 43 and 64% for the NBC112 peptide and specific yields of 36, 46 and 78% for the NBC759 peptide with the three protocols, respectively. Each protocol has different application contexts with advantages and disadvantages in each case. Conclusions: The three protocols allow the obtention of the two peptides with good purity and can be used according to specific needs and requirements.This article includes an interactive 360-degree video. To view it correctly, it is necessary to scroll through the screen to navigate across the laboratory where you will find 10 interactive points. For an immersive experience, a head-mounted display can be used. Please, visit this URL: http://ejbiotechnology.info/public/360view/2023/VTPCARDENAS1v1/index.html.How to cite: Guzmán F, Aróstica M, Román T, et al. Peptides, solid-phase synthesis and characterization: Tailor-made methodologies. Electron J Biotechnol 2023;64. https://doi.org/10.1016/j.ejbt.2023.01.005.

Keywords

• Automated peptide synthesis
• Manual peptide synthesis
• Microwave
• Peptide purity
• Peptide synthesis protocols
• Peptide yield