Mucin 21 confers resistance to apoptosis in an O-glycosylation-dependent manner

Yuan Tian; Kaori Denda-Nagai; Tatsuya Tsukui; Katrin B. Ishii-Schrade; Kyoko Okada; Yoshihiro Nishizono; Kosuke Matsuzaki; Margarete Hafley; Robert S. Bresalier; Tatsuro Irimura

doi:10.1038/s41420-022-01006-4

Cell Death Discovery (Apr 2022)

Mucin 21 confers resistance to apoptosis in an O-glycosylation-dependent manner

Yuan Tian,
Kaori Denda-Nagai,
Tatsuya Tsukui,
Katrin B. Ishii-Schrade,
Kyoko Okada,
Yoshihiro Nishizono,
Kosuke Matsuzaki,
Margarete Hafley,
Robert S. Bresalier,
Tatsuro Irimura

Affiliations

Yuan Tian: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Kaori Denda-Nagai: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Tatsuya Tsukui: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Katrin B. Ishii-Schrade: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Kyoko Okada: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Yoshihiro Nishizono: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Kosuke Matsuzaki: Division of Glycobiologics, Intractable Disease Research Center, Juntendo University
Margarete Hafley: Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center
Robert S. Bresalier: Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center
Tatsuro Irimura: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo

DOI: https://doi.org/10.1038/s41420-022-01006-4
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

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Mucin 21 (MUC21) is a large glycoprotein that protects squamous epithelia. Glycan changes in mucins occur in cancer cells and are thought to contribute to malignant progression. We report glycoform-dependent antiapoptotic effects of MUC21. Various MUC21 glycoforms were expressed in HEK293 and CHO cells. Apoptosis was induced using etoposide or UV exposure. MUC21 with glycans terminated with galactose/sialic acid inhibited apoptosis; MUC21 with no glycans or N-acetylgalactoseamine did not.

Published in Cell Death Discovery

ISSN: 2058-7716 (Online)
Publisher: Nature Publishing Group
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Science: Biology (General): Cytology
Website: http://www.nature.com/cddiscovery/

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