Molecular detection of virulence genes for multi drug resistance Klebseilla pneumoniae in patients with urinary tract infections, Thi-Qar Provenance, Iraq

Abstract

An agent that contributes to hospital acquired illnesses is Klebsiella pneumoniae. In community-acquired infections and illnesses of the urinary tract, it is the primary and most frequent causal agent. The strains were detected by using Analytical Profile Index (Api-20 E) and 16s rRNA techniques, fifteen trains of K. pneumoniae were obtained from eighteen urine samples collected from patients in Al-Hussein Hospital, Thi-Qar Governorate, Iraq. This research sought to identify the antibiotic resistance of K. pneumoniae to Aztreonam, Ceftriaxone, Trimethoprim, Amikacin, Ciprofloxacin, Meropenem, and Piperacillin, the resistance was high and ranged between (86.6-100)%. The multidrug resistance percentage was 100%, in addition the investigation aimed to detect some virulence genes, (i.e. HtrA and MrkA gene) in K. pneumoniae. The HtrA and MrkA genes were found using the PCR method, which revealed that 6 (40%) of the isolates had the HtrA gene and that all strains under study lacked the MrkA gene.

Keywords

• mdr
• klebsiella pneumoniae
• urinary tract infections
• htra gene
• mrka gene