STUDY ON CRYOPRESERVATION OF PRIMARY GOAT KIDNEY CELL AND ITS SUSCEPTIBILITY TO INFECTION IN VITRO

Abstract

Primary goat kidney cells are used for isolation, propagation and production of vaccine against goat pox virus. Availability of kid at desired time is a major constraint for uninterrupted vaccine production. Keeping this in mind, this study was designed to standardize a method of preservation of primary goat kidney cell for a long period. In the present study, culture, maintenance and preservation of primary and secondary goat kidney cell were conducted and was observed that primary goat kidney cell can be successfully preserved in liquid nitrogen (-1960 C) for a period of one year. After each revival, the cryo-preserved cell could be propagated and the confluent monolayer of primary kid kidney cells was given infection with Goat Pox virus at 0.1 m.o.i. based on predetermined TCID50 of virus. The susceptibility of revived cells to Goat Pox virus infection was studied by characteristic cytopathic effect and the identity of the viral DNA in infected cell lysate was confirmed by PCR upto the cells of 9th subculture after revival.

Keywords

• Cryopreservation
• Primary goat kidney cell
• Susceptibility
• Infection
• PCR
• In vitro