Macrophages-derived high-mobility group box-1 protein induces endothelial progenitor cells pyroptosis

Menghao Zeng; Guibin Liang; Fangfang Yuan; Shanshan Yan; Jie Liu; Zhihui He

iScience (Oct 2024)

Macrophages-derived high-mobility group box-1 protein induces endothelial progenitor cells pyroptosis

Menghao Zeng,
Guibin Liang,
Fangfang Yuan,
Shanshan Yan,
Jie Liu,
Zhihui He

Affiliations

Menghao Zeng: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China
Guibin Liang: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China
Fangfang Yuan: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Shanshan Yan: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Jie Liu: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Zhihui He: Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China; National Engineering Research Center for Human Stem Cells, Changsha, Hunan, China; Corresponding author

Journal volume & issue: Vol. 27, no. 10
p. 110996

Abstract

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Summary: Endothelial dysfunction is an important factor in the progress of sepsis. Endothelial progenitor cells (EPCs) are the precursor cells of endothelial cells and play a crucial role in the prognosis and treatment of sepsis. EPCs in the peripheral blood of patients with sepsis undergo pyroptosis, but the mechanism remains much of unknown. Serum high-mobility group box-1 (HMGB1) is significantly elevated in patients with sepsis, but whether it is related to EPCs pyroptosis is unknown. We used a cell model of sepsis in vitro to isolate EPCs for better observation. By detecting the pyroptosis-related indicators of EPCs and the level of release and acetylation of HMGB1 in inflammatory macrophages, it was found that HMGB1 released by inflammatory macrophages combined with receptor for advanced glycation end products (RAGE) is a key pathway to induce pyroptosis of EPCs.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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