eLife (Jun 2019)
Circulating T cell-monocyte complexes are markers of immune perturbations
- Julie G Burel,
- Mikhail Pomaznoy,
- Cecilia S Lindestam Arlehamn,
- Daniela Weiskopf,
- Ricardo da Silva Antunes,
- Yunmin Jung,
- Mariana Babor,
- Veronique Schulten,
- Gregory Seumois,
- Jason A Greenbaum,
- Sunil Premawansa,
- Gayani Premawansa,
- Ananda Wijewickrama,
- Dhammika Vidanagama,
- Bandu Gunasena,
- Rashmi Tippalagama,
- Aruna D deSilva,
- Robert H Gilman,
- Mayuko Saito,
- Randy Taplitz,
- Klaus Ley,
- Pandurangan Vijayanand,
- Alessandro Sette,
- Bjoern Peters
Affiliations
- Julie G Burel
- ORCiD
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Mikhail Pomaznoy
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Cecilia S Lindestam Arlehamn
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Daniela Weiskopf
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Ricardo da Silva Antunes
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Yunmin Jung
- Division of Inflammation Biology, La Jolla Institute for Immunology, La Jolla, United States
- Mariana Babor
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Veronique Schulten
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Gregory Seumois
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States
- Jason A Greenbaum
- Bioinformatics core, La Jolla Institute for Immunology, La Jolla, United States
- Sunil Premawansa
- Department of Zoology and Environment Sciences, Science Faculty, University of Colombo, Colombo, Sri Lanka
- Gayani Premawansa
- North Colombo Teaching Hospital, Ragama, Sri Lanka
- Ananda Wijewickrama
- National Institute of Infectious Diseases, Gothatuwa, Sri Lanka
- Dhammika Vidanagama
- National Tuberculosis Reference Laboratory, Welisara, Sri Lanka
- Bandu Gunasena
- National Hospital for Respiratory Diseases, Welisara, Sri Lanka
- Rashmi Tippalagama
- Genetech Research Institute, Colombo, Sri Lanka
- Aruna D deSilva
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States; Genetech Research Institute, Colombo, Sri Lanka
- Robert H Gilman
- Johns Hopkins School of Public Health, Baltimore, United States; Universidad Peruana Cayetano Heredia, Lima, Peru
- Mayuko Saito
- Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan
- Randy Taplitz
- Division of Infectious Diseases and Global Public Health, University of California, San Diego, La Jolla, United States
- Klaus Ley
- Division of Inflammation Biology, La Jolla Institute for Immunology, La Jolla, United States; Department of Bioengineering, University of California, San Diego, La Jolla, United States
- Pandurangan Vijayanand
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States; Department of Medicine, University of California, San Diego, La Jolla, United States
- Alessandro Sette
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States; Department of Medicine, University of California, San Diego, La Jolla, United States
- Bjoern Peters
- ORCiD
- Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, United States; Department of Medicine, University of California, San Diego, La Jolla, United States
- DOI
- https://doi.org/10.7554/eLife.46045
- Journal volume & issue
-
Vol. 8
Abstract
Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artifacts and thus ignored in data acquisition and analysis, are the result of biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be re-visited.
Keywords
