Química Nova (Jan 2012)

Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

  • Raquel D. C. C. Bandeira,
  • Thais M. Uekane,
  • Carolina P. da Cunha,
  • Luths R. O. Geaquinto,
  • Valnei S. Cunha,
  • Janaina M. R. Caixeiro,
  • Ronoel Luiz O. Godoy,
  • Marcus Henrique C. de la Cruz

DOI
https://doi.org/10.1590/S0100-40422012000100013
Journal volume & issue
Vol. 35, no. 1
pp. 66 – 71

Abstract

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A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.

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