EMBO Molecular Medicine (Aug 2018)
Comparative proteomics reveals a diagnostic signature for pulmonary head‐and‐neck cancer metastasis
- Hanibal Bohnenberger,
- Lars Kaderali,
- Philipp Ströbel,
- Diego Yepes,
- Uwe Plessmann,
- Neekesh V Dharia,
- Sha Yao,
- Carina Heydt,
- Sabine Merkelbach‐Bruse,
- Alexander Emmert,
- Jonatan Hoffmann,
- Julius Bodemeyer,
- Kirsten Reuter‐Jessen,
- Anna‐Maria Lois,
- Leif Hendrik Dröge,
- Philipp Baumeister,
- Christoph Walz,
- Lorenz Biggemann,
- Roland Walter,
- Björn Häupl,
- Federico Comoglio,
- Kuan‐Ting Pan,
- Sebastian Scheich,
- Christof Lenz,
- Stefan Küffer,
- Felix Bremmer,
- Julia Kitz,
- Maren Sitte,
- Tim Beißbarth,
- Marc Hinterthaner,
- Martin Sebastian,
- Joachim Lotz,
- Hans‐Ulrich Schildhaus,
- Hendrik Wolff,
- Bernhard C Danner,
- Christian Brandts,
- Reinhard Büttner,
- Martin Canis,
- Kimberly Stegmaier,
- Hubert Serve,
- Henning Urlaub,
- Thomas Oellerich
Affiliations
- Hanibal Bohnenberger
- Institute of Pathology, University Medical Center
- Lars Kaderali
- Institute of Bioinformatics, University Medicine Greifswald
- Philipp Ströbel
- Institute of Pathology, University Medical Center
- Diego Yepes
- Department of Medicine II, Hematology/Oncology, Goethe University
- Uwe Plessmann
- Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
- Neekesh V Dharia
- Department of Pediatric Oncology, Dana‐Farber Cancer Institute
- Sha Yao
- Institute of Pathology, University Medical Center
- Carina Heydt
- Institute of Pathology, University Hospital Cologne, University of Cologne
- Sabine Merkelbach‐Bruse
- Institute of Pathology, University Hospital Cologne, University of Cologne
- Alexander Emmert
- Department of Thoracic and Cardiovascular Surgery, University Medical Center
- Jonatan Hoffmann
- Institute of Pathology, University Medical Center
- Julius Bodemeyer
- Institute of Pathology, University Medical Center
- Kirsten Reuter‐Jessen
- Institute of Pathology, University Medical Center
- Anna‐Maria Lois
- Institute of Pathology, University Medical Center
- Leif Hendrik Dröge
- Department of Radiooncology, University Medical Center
- Philipp Baumeister
- Department of Otorhinolaryngology, University Hospital Munich, Ludwig‐Maximilian‐University München
- Christoph Walz
- Institute of Pathology, Faculty of Medicine, LMU Munich
- Lorenz Biggemann
- Institute for Diagnostic and Interventional Radiology, University Medical Center
- Roland Walter
- Department of Medicine II, Hematology/Oncology, Goethe University
- Björn Häupl
- Department of Medicine II, Hematology/Oncology, Goethe University
- Federico Comoglio
- Department of Haematology, University of Cambridge
- Kuan‐Ting Pan
- Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
- Sebastian Scheich
- Department of Medicine II, Hematology/Oncology, Goethe University
- Christof Lenz
- Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
- Stefan Küffer
- Institute of Pathology, University Medical Center
- Felix Bremmer
- Institute of Pathology, University Medical Center
- Julia Kitz
- Institute of Pathology, University Medical Center
- Maren Sitte
- Institute of Medical Statistics, University Medical Center
- Tim Beißbarth
- Institute of Medical Statistics, University Medical Center
- Marc Hinterthaner
- Department of Thoracic and Cardiovascular Surgery, University Medical Center
- Martin Sebastian
- Department of Medicine II, Hematology/Oncology, Goethe University
- Joachim Lotz
- Institute for Diagnostic and Interventional Radiology, University Medical Center
- Hans‐Ulrich Schildhaus
- Institute of Pathology, University Medical Center
- Hendrik Wolff
- University Medical Center
- Bernhard C Danner
- Department of Thoracic and Cardiovascular Surgery, University Medical Center
- Christian Brandts
- Department of Medicine II, Hematology/Oncology, Goethe University
- Reinhard Büttner
- Institute of Pathology, University Hospital Cologne, University of Cologne
- Martin Canis
- Department of Otorhinolaryngology, University Hospital Munich, Ludwig‐Maximilian‐University München
- Kimberly Stegmaier
- Department of Pediatric Oncology, Dana‐Farber Cancer Institute
- Hubert Serve
- Department of Medicine II, Hematology/Oncology, Goethe University
- Henning Urlaub
- Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
- Thomas Oellerich
- Department of Medicine II, Hematology/Oncology, Goethe University
- DOI
- https://doi.org/10.15252/emmm.201708428
- Journal volume & issue
-
Vol. 10,
no. 9
pp. 1 – 15
Abstract
Abstract Patients with head‐and‐neck cancer can develop both lung metastasis and primary lung cancer during the course of their disease. Despite the clinical importance of discrimination, reliable diagnostic biomarkers are still lacking. Here, we have characterised a cohort of squamous cell lung (SQCLC) and head‐and‐neck (HNSCC) carcinomas by quantitative proteomics. In a training cohort, we quantified 4,957 proteins in 44 SQCLC and 30 HNSCC tumours. A total of 518 proteins were found to be differentially expressed between SQCLC and HNSCC, and some of these were identified as genetic dependencies in either of the two tumour types. Using supervised machine learning, we inferred a proteomic signature for the classification of squamous cell carcinomas as either SQCLC or HNSCC, with diagnostic accuracies of 90.5% and 86.8% in cross‐ and independent validations, respectively. Furthermore, application of this signature to a cohort of pulmonary squamous cell carcinomas of unknown origin leads to a significant prognostic separation. This study not only provides a diagnostic proteomic signature for classification of secondary lung tumours in HNSCC patients, but also represents a proteomic resource for HNSCC and SQCLC.
Keywords
