Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model

Bum Soo Kim; So Young Chun; Eun Hye Lee; Jae-Wook Chung; Jun Nyung Lee; Yun-Sok Ha; Jae Young Choi; Phil Hyun Song; Tae Gyun Kwon; Man-Hoon Han; Dae Hwan Kim; Eun Sang Yoo

Stem Cell Research (May 2020)

Efficacy of combination therapy with pentosan polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model

Bum Soo Kim,
So Young Chun,
Eun Hye Lee,
Jae-Wook Chung,
Jun Nyung Lee,
Yun-Sok Ha,
Jae Young Choi,
Phil Hyun Song,
Tae Gyun Kwon,
Man-Hoon Han,
Dae Hwan Kim,
Eun Sang Yoo

Affiliations

Bum Soo Kim: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South Korea
So Young Chun: BioMedical Research Institute, Kyungpook National University Hospital, Daegu, South Korea
Eun Hye Lee: BioMedical Research Institute, Kyungpook National University Hospital, Daegu, South Korea
Jae-Wook Chung: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South Korea
Jun Nyung Lee: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South Korea
Yun-Sok Ha: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South Korea
Jae Young Choi: Department of Urology, Yeungnam University College of Medicine, Daegu, South Korea
Phil Hyun Song: Department of Urology, Yeungnam University College of Medicine, Daegu, South Korea
Tae Gyun Kwon: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, South Korea
Man-Hoon Han: Department of Pathology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South Korea
Dae Hwan Kim: Department of Laboratory Animal Research Support Team, Yeungnam University Hospital, Daegu, South Korea
Eun Sang Yoo: Department of Urology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, South Korea; Corresponding author. Address: Department of Urology, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, Korea. Postal code: 41944. Telephone: 82 53 2005851; Fax: 82-53-421-9618.

Journal volume & issue: Vol. 45

Abstract

Read online

We evaluated the synergistic effects of pentosan polysulfate sodium (PPS) and mesenchymal stem cells (MSCs) in an interstitial cystitis (IC) rat model. After generation of the IC rat model, the rats were divided into 4 groups according to the treatment they received: phosphate-buffered saline injection into bladder submucosa, daily oral PPS feeding, MSC injection into bladder submucosa, or MSC injection into bladder submucosa with daily oral PPS feeding. After treatment, conscious cystometry and pain scale measurement were performed and their bladders were obtained for histological and proinflammatory-related gene expression analysis. On cystometric analysis, all treatment groups showed significantly increased intercontraction intervals and lower pain scores compared to those of the control group. Histological analysis revealed regenerated urothelium, less fibrosis, and decreased mast cell infiltration in all treatment groups compared to the control group. Significantly lower expression of TNF-α, IFN-γ, MCP, IL-6, TLR2, and TLR11 was observed in the PPS with MSC group compared to the other groups. Combination therapy with PPS and MSCs showed histological and functional effects in an IC rat model, including synergistic effects leading to increased intercontraction interval and decreased inflammatory reactions.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal

Abstract

Keywords