Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries

Kabo Masisi; Riach Masamba; Keletso Lashani; Chunyang Li; Tebogo E. Kwape; Goabaone Gaobotse

doi:10.3831/KPI.2021.24.1.24

Journal of Pharmacopuncture (Mar 2021)

Antioxidant, Cytotoxicity and Cytoprotective Potential of Extracts of Grewia Flava and Grewia Bicolor Berries

Kabo Masisi,
Riach Masamba,
Keletso Lashani,
Chunyang Li,
Tebogo E. Kwape,
Goabaone Gaobotse

Affiliations

Kabo Masisi: Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana
Riach Masamba: Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana
Keletso Lashani: Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana
Chunyang Li: Department of Nutrition and Health, Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China
Tebogo E. Kwape: Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana
Goabaone Gaobotse: Department of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, Botswana

DOI: https://doi.org/10.3831/KPI.2021.24.1.24
Journal volume & issue: Vol. 24, no. 1
pp. 24 – 31

Abstract

Read online

Objectives: Accumulation of cellular reactive oxygen species (ROS) leads to oxidative stress. Increased production of ROS, such as superoxide anion, or a deficiency in their clearance by antioxidant defences, mediates cellular pathology. Grewia Spp fruits are a source of bioactive compounds and have notable antioxidant activity. Although the antioxidant capacity of Grewia Spp has been studied, there is very limited evidence that links the antioxidant activities of Grewia bicolor and Grewia flava to the inhibition of free radical formation associated with damage in biological systems.Methods : : This study evaluated the protective effects of Grewia bicolor and Grewia flava extracts against free radical-induced oxidative stress and the resulting cytotoxicity effect using HeLa cells. Antioxidant properties determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total phenolic content (TPC) assays showed significantly higher (p < 0.05) antioxidant activity in Grewia flava (ethanol extract) than Grewia flava (water extract) and Grewia bicolor (ethanol and water extracts).Results : : Using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide or MTT assay, cytotoxicity results showed that extracts of Grewia bicolor and Grewia flava were less toxic to HeLa cells at tested concentrations compared to the untreated control. This confirmed the low toxicity of these edible fruits at the tested concentrations in HeLa cells. Furthermore, hydrogen peroxide (H2O2)-induced cell loss was effectively reduced by pre-incubating HeLa cells with Grewia bicolor and Grewia flava extracts, with Grewia flava (ethanol extract) revealing better protection.Conclusion : : The effect was speculated to be associated with the higher antioxidant activity of Grewia flava (ethanol extract). Additional studies will warrant confirmation of the mechanism of action of such effects.

Published in Journal of Pharmacopuncture

ISSN: 2093-6966 (Print); 2234-6856 (Online)
Publisher: Korean Pharmacopuncture Institute
Country of publisher: Korea, Republic of
LCC subjects: Medicine: Other systems of medicine: Miscellaneous systems and treatments; Medicine: Therapeutics. Pharmacology
Website: http://www.journal-jop.org/main.html

About the journal

Abstract

Keywords