Potency of mycotoxin binders on MDA level, expressions of caspase 9 and caspase 3 in the uterus of mice exposed to zearalenone

Abstract

This study examined effect of mycotoxin binder administered to female mice exposed to zearalenon on apoptosis incidence by observe at MDA, Caspase 9 and 3 levels in mice uterus. Negative control group (K-) was not exposed to zearalenon and without the administration of mycotoxin binders, positive control group (K+) exposed to zearalenon of 0.1 mg/head/day and without the provision of mycotoxin binders; and treatment groups (P1, P2, P3) were exposed to zearalenon 0.1 mg/head/day by providing mycotoxin binders each 0.5; 1; 2 mg/head/day. Zearalenon and mycotoxinbinders administration was conducted for 10 days. Results on MDA level were as follows 15.48 ± 0.50 (K-), 45.59 ± 0.50 (K+), 34.92 ± 3.38 (P1), 27.72 ± 1.25 and 23.89 ± 3.74. Caspase 9 levels showed the following results: 0.3 ± 0.60 (K), 8.3 ± 0.90 (K+), 3.6 ± 0.41 (P1), 3.3 ± 0.34 (P2) and 2.8 ± 0.28 (P3), while the levels of Caspase3 were as follows: 3.35 ± 0.44 (K-), 12.5 ± 0.66 (K+), 3.6 ± 0.41 (P1), 4.80 ± 0.43 (P2) and 3.85 ± 0.50 (P3). In conclusion, mycotoxin binders may lower malondialdehyde (MDA) level and the expression of caspase 9 and caspase3 in the uterus of mice exposed to zearalenon.

Keywords

• zearalenone
• mycotoxin binders
• mda level
• caspase