Development and Applications of Enhanced Green Fluorescent Protein Mutants

R.H. Stauber; K. Horie; P. Carney; E.A. Hudson; N.I. Tarasova; G.A. Gaitanaris; G.N. Pavlakis

doi:10.2144/98243rr01

BioTechniques (Mar 1998)

Development and Applications of Enhanced Green Fluorescent Protein Mutants

R.H. Stauber,
K. Horie,
P. Carney,
E.A. Hudson,
N.I. Tarasova,
G.A. Gaitanaris,
G.N. Pavlakis

Affiliations

R.H. Stauber: 1National Cancer Institute-FCRDC, Frederick, MD, USA
K. Horie: 1National Cancer Institute-FCRDC, Frederick, MD, USA
P. Carney: 1National Cancer Institute-FCRDC, Frederick, MD, USA
E.A. Hudson: 1National Cancer Institute-FCRDC, Frederick, MD, USA
N.I. Tarasova: 1National Cancer Institute-FCRDC, Frederick, MD, USA
G.A. Gaitanaris: 1National Cancer Institute-FCRDC, Frederick, MD, USA
G.N. Pavlakis: 1National Cancer Institute-FCRDC, Frederick, MD, USA

DOI: https://doi.org/10.2144/98243rr01
Journal volume & issue: Vol. 24, no. 3
pp. 462 – 471

Abstract

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The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

Published in BioTechniques

ISSN: 0736-6205 (Print); 1940-9818 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.tandfonline.com/journals/ibtn20

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