Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold

Abstract

The main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the optimal cross-linking concentration and gelation temperature were determined. Secondly, a prediction model of the extrusion line width of SA-GEL was established, in which the printing pressure, the moving speed of the needle and the fiber interval were the important parameters affecting the printing performance of the SA-GEL composite material. Thirdly, the SA-GEL composite scaffolds were printed on the Bio-plotter platform, the C5.18 chondrocytes cells were cultured in the SA-GEL biomaterial scaffold, and the results show that the cells could survive well. These results show that, under the control of the printing parameters pressure 1.8 bar, moving speed 10.7 mm/s and the internal structure parameters of the scaffold is 0/45-1.2 (Printing interval: 1.2 mm, angle value: 45 degree), SA-GEL scaffold printing results can be obtained which have good mechanical properties and biocompatibility.

Keywords

• biological 3d printing
• sodium alginate
• gelatin
• cartilage scaffold