Forensic Sciences (Sep 2024)

A Reflective Spectroscopy Proof-of-Concept Study of Urea for Supporting Investigations of Human Waste in Multiple Forensic Contexts

  • Lilly McClelland,
  • Ethan Belak,
  • Juliana Curtis,
  • Ethan Krekeler,
  • April Sanders,
  • Mark P. S. Krekeler

DOI
https://doi.org/10.3390/forensicsci4030030
Journal volume & issue
Vol. 4, no. 3
pp. 463 – 474

Abstract

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Human urine and its detection are of interest in forensic studies in numerous contexts. Both crystalline urea and 1.0 M solutions of urea, as synthetic analog endmember components of human urine, were investigated as a proof-of-concept study to determine if detailed lab spectroscopy would be viable. Urea was reliably detected on Ottawa sand at concentrations of approximately 3.2% in dried experiments. Urea was detectable after 1 week of solution evaporation under lab conditions, at 9.65 wt.% 1 M solution. This investigation successfully establishes urea as a material of interest for reflective spectroscopy and hyperspectral remote sensing/image spectroscopy on a wide range of spatial scales, from specific centimeter-scale areas in a crime scene to searching large outdoor regions > 1 km2. In addition, this investigation is relevant to improving the monitoring of human trafficking, status and condition of refugee camps, and monitoring sewage.

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