Purification of micronuclei from cultured cells by flow cytometry

Abstract

Summary: Micronuclei are aberrant nuclear compartments that form when chromosomes or chromosome fragments fail to incorporate into a primary nucleus during mitotic exit. Ruptures at the micronuclear envelope are associated with DNA damage and activation of immune sensing pathways. To gain insights into these processes, we have developed a method to purify ruptured micronuclei. This method paves the way toward understanding the consequences of micronuclear envelope rupture.For complete details on the use and execution of this protocol, please refer to Mohr et al. (2021).

Keywords

• Cell culture
• Flow cytometry/mass cytometry
• Immunology
• Molecular biology