pEGFP/Ang-1 transfected BMSCs attenuates glucose-damaged RF/6A cells

Abstract

AIM:To investigate the protective effects of pEGFP/ Ang-1 transfected BMSCs on RF/6A in condition of high glucose concentration. METHODS: BMSCs were transfected pEGFP/Ang-1, confirming by observation of enhanced green fluorescent protein(EGFP)with fluorescence microscope. Rhesus chorioido-retinal endothelial cells(RF/6A)were cultured with or without pEGFP/Ang-1 transfected BMSCs in transwell under high glucose concentration. Three days after co-cultured, cell vitality and pPKB expression of RF/6A were detected with methyl thiazolyl tetrazolium(MTT)methods and Western blot respectively. RESULTS: EGFP was expressed in BMSCs successfully transfected with pEGFP/Ang-1. RF/6A co-cultured with pEGFP/Ang-1 transfected BMSCs showed higher viability and pPKB expression compared with RF/6A cultured in condition of high glucose concentration cultured with or without untransfected BMSCs(PP> 0.05 respectively).CONCLUSION: pEGFP/Ang-1 transfected BMSCs showed protective effects, may via PKB, on RF/6A in condition of high glucose concentration.

Keywords

• angiopoietin-1
• bone marrow mesenchymal stem cells
• Rhesus choroidal-retinal vascular endothelial cell
• high glucose concentration
• high glucose concentration