Frontiers in Oncology (Apr 2021)

Comprehensive and Computable Molecular Diagnostic Panel (C2Dx) From Small Volume Specimens for Precision Oncology: Molecular Subtyping of Non-Small Cell Lung Cancer From Fine Needle Aspirates

  • Jing Su,
  • Jing Su,
  • Jing Su,
  • Lynn S. Huang,
  • Ryan Barnard,
  • Graham Parks,
  • James Cappellari,
  • Christina Bellinger,
  • Travis Dotson,
  • Lou Craddock,
  • Bharat Prakash,
  • Jonathan Hovda,
  • Hollins Clark,
  • William Jeffrey Petty,
  • Boris Pasche,
  • Michael D. Chan,
  • Lance D. Miller,
  • Jimmy Ruiz,
  • Jimmy Ruiz

DOI
https://doi.org/10.3389/fonc.2021.584896
Journal volume & issue
Vol. 11

Abstract

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The Comprehensive, Computable NanoString Diagnostic gene panel (C2Dx) is a promising solution to address the need for a molecular pathological research and diagnostic tool for precision oncology utilizing small volume tumor specimens. We translate subtyping-related gene expression patterns of Non-Small Cell Lung Cancer (NSCLC) derived from public transcriptomic data which establish a highly robust and accurate subtyping system. The C2Dx demonstrates supreme performance on the NanoString platform using microgram-level FNA samples and has excellent portability to frozen tissues and RNA-Seq transcriptomic data. This workflow shows great potential for research and the clinical practice of cancer molecular diagnosis.

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