Comparative Transcriptome Analysis of Tolerant and Sensitive Genotypes of Common Bean (<i>Phaseolus vulgaris</i> L.) in Response to Terminal Drought Stress

Mayavan Subramani; Carlos A. Urrea; Rasheed Habib; Ketaki Bhide; Jyothi Thimmapuram; Venu Kalavacharla

doi:10.3390/plants12010210

Plants (Jan 2023)

Comparative Transcriptome Analysis of Tolerant and Sensitive Genotypes of Common Bean (<i>Phaseolus vulgaris</i> L.) in Response to Terminal Drought Stress

Mayavan Subramani,
Carlos A. Urrea,
Rasheed Habib,
Ketaki Bhide,
Jyothi Thimmapuram,
Venu Kalavacharla

Affiliations

Mayavan Subramani: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA
Carlos A. Urrea: Panhandle Research and Extension Center, University of Nebraska, 4502 Avenue I, Scottsbluff, NE 69361, USA
Rasheed Habib: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA
Ketaki Bhide: Bioinformatics Core, Purdue University, West Lafayette, IN 47907, USA
Jyothi Thimmapuram: Bioinformatics Core, Purdue University, West Lafayette, IN 47907, USA
Venu Kalavacharla: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA

DOI: https://doi.org/10.3390/plants12010210
Journal volume & issue: Vol. 12, no. 1
p. 210

Abstract

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We conducted a genome-wide transcriptomic analysis of three drought tolerant and sensitive genotypes of common bean to examine their transcriptional responses to terminal drought stress. We then conducted pairwise comparisons between the root and leaf transcriptomes from the resulting tissue based on combined transcriptomic data from the tolerant and sensitive genotypes. Our transcriptomic data revealed that 491 (6.4%) DEGs (differentially expressed genes) were upregulated in tolerant genotypes, whereas they were downregulated in sensitive genotypes; likewise, 396 (5.1%) DEGs upregulated in sensitive genotypes were downregulated in tolerant genotypes. Several transcription factors, heat shock proteins, and chaperones were identified in the study. Several DEGs in drought DB (data Base) overlapped between genotypes. The GO (gene ontology) terms for biological processes showed upregulation of DEGs in tolerant genotypes for sulfate and drug transmembrane transport when compared to sensitive genotypes. A GO term for cellular components enriched with upregulated DEGs for the apoplast in tolerant genotypes. These results substantiated the temporal pattern of root growth (elongation and initiation of root growth), and ABA-mediated drought response in tolerant genotypes. KEGG (kyoto encyclopedia of genes and genomes) analysis revealed an upregulation of MAPK (mitogen activated protein kinase) signaling pathways and plant hormone signaling pathways in tolerant genotypes. As a result of this study, it will be possible to uncover the molecular mechanisms of drought tolerance in response to terminal drought stress in the field. Further, genome-wide transcriptomic analysis of both tolerant and sensitive genotypes will assist us in identifying potential genes that may contribute to improving drought tolerance in the common bean.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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