Известия высших учебных заведений. Поволжский регион: Естественные науки (Mar 2021)
Effect of the water deficit on very long chain fatty acids in barley seedlings leaves
Abstract
Background. The obtained data indicate the potential role of fatty acids with very long chain (VLCFA) as signaling molecules in the management of both biotic and abiotic stress, including osmotic stress. The purpose of the research is to study the effect of the electromagnetic microwave field (EMF) and osmotic stress on the change of the RFC profile in the leaves of barley seedlings. Materials and methods. The research was carried out on monthly barley seedlings grown in soil culture in a mixture of peat and sand under laboratory conditions. The scheme of the experiment included six options: 1) control, without microwave treatment, normal humidification; 2) microwave treatment with a power of 0,42 kW, frequency of 2,45 GHz, with an exposure of 11 seconds, normal humidification; 3) microwave treatment with a power of 0,70 kW, frequency of 2,45 GHz, with an exposure of 11 seconds, normal humidification; 4–6) the same options, but grown in conditions of water scarcity. The content of fatty acids was determined by mass spectrometry. Results. The effect of electromagnetic fields with a power of 0,42 kW when growing barley seedlings under normal water supply conditions in relation to growing them in conditions of water scarcity was manifested in an increase in the content of erucic acid by 1,6 times, and high power (0,70 kW) – by 1,9 times. The increase in the content of arachidic and begenic fatty acids. It is shown that the combined effect of microwave EMF and water deficiency changes the profile of fatty acids with a very long chain, which is expressed in a significant increase in erucic acid in all experimental variants. Conclusions. When the plant body is affected by such abiotic factors as drought and an ultra-high frequency electromagnetic field of medium and high power, a change in the profile of fatty acids with a very long chain is observed. Changes in the profile of the VLCFA in leaves under the influence of water stress and EMF are associated with the activation of the main adaptive systems in the body of barley seedlings.
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