Communications Biology (Jan 2021)
The spatial RNA integrity number assay for in situ evaluation of transcriptome quality
- Linda Kvastad,
- Konstantin Carlberg,
- Ludvig Larsson,
- Eva Gracia Villacampa,
- Alexander Stuckey,
- Linnea Stenbeck,
- Annelie Mollbrink,
- Margherita Zamboni,
- Jens Peter Magnusson,
- Elisa Basmaci,
- Alia Shamikh,
- Gabriela Prochazka,
- Anna-Lena Schaupp,
- Åke Borg,
- Lars Fugger,
- Monica Nistér,
- Joakim Lundeberg
Affiliations
- Linda Kvastad
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Konstantin Carlberg
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Ludvig Larsson
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Eva Gracia Villacampa
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Alexander Stuckey
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Linnea Stenbeck
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Annelie Mollbrink
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- Margherita Zamboni
- Department of Cell and Molecular Biology, Karolinska Institutet
- Jens Peter Magnusson
- Department of Cell and Molecular Biology, Karolinska Institutet
- Elisa Basmaci
- Department of Oncology-Pathology, Karolinska Institutet
- Alia Shamikh
- Department of Oncology-Pathology, Karolinska Institutet
- Gabriela Prochazka
- Department of Oncology-Pathology, Karolinska Institutet
- Anna-Lena Schaupp
- Nuffield Department of Clinical Neurosciences, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital University of Oxford, Oxford Centre for Neuroinflammation
- Åke Borg
- Division of Oncology and Pathology, Department of Clinical Sciences, Lund Lund University
- Lars Fugger
- Nuffield Department of Clinical Neurosciences, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital University of Oxford, Oxford Centre for Neuroinflammation
- Monica Nistér
- Department of Oncology-Pathology, Karolinska Institutet
- Joakim Lundeberg
- Science for Life Laboratory, KTH - Royal Institute of Technology (KTH), SE-171 65
- DOI
- https://doi.org/10.1038/s42003-020-01573-1
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 8
Abstract
Kvastad et al. develop the spatial RNA Integrity Number (sRIN) assay that evaluates the RNA integrity at cellular resolution. This method improves the resolution of a similar method called the RNA Integrity Number (RIN), demonstrating spatial variation in the quality of RNA samples.
