Frontiers in Cellular and Infection Microbiology (Feb 2024)

Transmission dynamics of drug-resistant tuberculosis in Ningbo, China: an epidemiological and genomic analysis

  • Yang Che,
  • Xiangchen Li,
  • Tong Chen,
  • Yewei Lu,
  • Guoxin Sang,
  • Junli Gao,
  • Junshun Gao,
  • Zhengwei Liu,
  • Tianfeng He,
  • Yi Chen

DOI
https://doi.org/10.3389/fcimb.2024.1327477
Journal volume & issue
Vol. 14

Abstract

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BackgroundTuberculosis (TB), particularly drug-resistant TB (DR-TB), remains a significant public health concern in Ningbo, China. Understanding its molecular epidemiology and spatial distribution is paramount for effective control.MethodsFrom December 24, 2020, to March 12, 2023, we collected clinical Mycobacterium tuberculosis (MTB) strains in Ningbo, with whole-genome sequencing performed on 130 MTB strains. We analyzed DR-related gene mutations, conducted phylogenetic and phylodynamic analyses, identified recent transmission clusters, and assessed spatial distribution.ResultsAmong 130 DR-TB cases, 41% were MDR-TB, 36% pre-XDR-TB, 19% RR-TB, and 3% HR-TB. The phylogenetic tree showed that 90% of strains were Lineage 2 (Beijing genotype), while remaining 10% were Lineage 4 (Euro-American genotype). The spatial analysis identified hotspots of DR-TB in Ningbo’s northern region, particularly in traditional urban centers. 31 (24%) of the DR-TB cases were grouped into 7 recent transmission clusters with a large outbreak cluster containing 15 pre-XDR-TB patients. Epidemiological analyses suggested a higher risk of recent DR-TB transmission among young adult patients who frequently visited Internet cafes, game rooms, and factories.ConclusionOur study provides comprehensive insights into the epidemiology and genetics of DR-TB in Ningbo. The presence of genomic clusters highlights recent transmission events, indicating the need for targeted interventions. These findings are vital for informing TB control strategies in Ningbo and similar settings.

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