Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

Rafael Molina; Stefano Stella; Mingxia Feng; Nicholas Sofos; Vykintas Jauniskis; Irina Pozdnyakova; Blanca López-Méndez; Qunxin She; Guillermo Montoya

doi:10.1038/s41467-019-12244-z

Nature Communications (Sep 2019)

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

Rafael Molina,
Stefano Stella,
Mingxia Feng,
Nicholas Sofos,
Vykintas Jauniskis,
Irina Pozdnyakova,
Blanca López-Méndez,
Qunxin She,
Guillermo Montoya

Affiliations

Rafael Molina: Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B
Stefano Stella: Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B
Mingxia Feng: College of Life Science and Technology, Huazhong Agricultural University
Nicholas Sofos: Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B
Vykintas Jauniskis: Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B
Irina Pozdnyakova: The Novo Nordisk Foundation Center for Protein Research, Protein Structure & Function Programme, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B
Blanca López-Méndez: The Novo Nordisk Foundation Center for Protein Research, Protein Structure & Function Programme, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B
Qunxin She: College of Life Science and Technology, Huazhong Agricultural University
Guillermo Montoya: Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B

DOI: https://doi.org/10.1038/s41467-019-12244-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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Type III CRISPR-Cas RNases from the Csm and Csx families are activated by cyclic oligoadenylates (cOA). Here the authors present the cOA bound Sulfolobus islandicus Csx1 structure, which forms a hexamer and reveal an allosteric mechanism for the activation of Csx1 RNase.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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