Reconstruction and Analysis of a Genome-Scale Metabolic Model of Ganoderma lucidum for Improved Extracellular Polysaccharide Production

Zhongbao Ma; Zhongbao Ma; Chao Ye; Chao Ye; Weiwei Deng; Mengmeng Xu; Mengmeng Xu; Qiong Wang; Qiong Wang; Gaoqiang Liu; Feng Wang; Liming Liu; Liming Liu; Zhenghong Xu; Zhenghong Xu; Guiyang Shi; Guiyang Shi; Zhongyang Ding; Zhongyang Ding

doi:10.3389/fmicb.2018.03076

Frontiers in Microbiology (Dec 2018)

Reconstruction and Analysis of a Genome-Scale Metabolic Model of Ganoderma lucidum for Improved Extracellular Polysaccharide Production

Zhongbao Ma,
Zhongbao Ma,
Chao Ye,
Chao Ye,
Weiwei Deng,
Mengmeng Xu,
Mengmeng Xu,
Qiong Wang,
Qiong Wang,
Gaoqiang Liu,
Feng Wang,
Liming Liu,
Liming Liu,
Zhenghong Xu,
Zhenghong Xu,
Guiyang Shi,
Guiyang Shi,
Zhongyang Ding,
Zhongyang Ding

Affiliations

Zhongbao Ma: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Zhongbao Ma: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Chao Ye: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Chao Ye: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Weiwei Deng: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Mengmeng Xu: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Mengmeng Xu: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Qiong Wang: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Qiong Wang: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Gaoqiang Liu: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China
Feng Wang: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China
Liming Liu: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Liming Liu: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Zhenghong Xu: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Zhenghong Xu: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Guiyang Shi: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Guiyang Shi: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China
Zhongyang Ding: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
Zhongyang Ding: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China

DOI: https://doi.org/10.3389/fmicb.2018.03076
Journal volume & issue: Vol. 9

Abstract

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In this study, we reconstructed for the first time a genome-scale metabolic model (GSMM) of Ganoderma lucidum strain CGMCC5.26, termed model iZBM1060, containing 1060 genes, 1202 metabolites, and 1404 reactions. Important findings based on model iZBM1060 and its predictions are as follows: (i) The extracellular polysaccharide (EPS) biosynthetic pathway was elucidated completely. (ii) A new fermentation strategy is proposed: addition of phenylalanine increased EPS production by 32.80% in simulations and by 38.00% in experiments. (iii) Eight genes for key enzymes were proposed for EPS overproduction. Model iZBM1060 provides a useful platform for regulating EPS production in terms of system metabolic engineering for G. lucidum, as well as a guide for future metabolic pathway construction of other high value-added edible/ medicinal mushroom species.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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