Frontiers in Microbiology (Oct 2018)
A Model for Trans-Kingdom Pathogenicity in Fonsecaea Agents of Human Chromoblastomycosis
- Gheniffer Fornari,
- Renata Rodrigues Gomes,
- Juliana Degenhardt-Goldbach,
- Suelen Silvana dos Santos,
- Sandro Rogério de Almeida,
- Germana Davila dos Santos,
- Marisol Dominguez Muro,
- Cleusa Bona,
- Rosana Herminia Scola,
- Edvaldo S. Trindade,
- Israel Henrique Bini,
- Lisandra Santos Ferreira-Maba,
- Daiane Rigoni Kestring,
- Mariana Machado Fidelis do Nascimento,
- Bruna Jacomel Favoreto de Souza Lima,
- Morgana F. Voidaleski,
- Douglas André Steinmacher,
- Bruna da Silva Soley,
- Shuwen Deng,
- Anamelia Lorenzetti Bocca,
- Moises B. da Silva,
- Claudio G. Salgado,
- Conceição Maria Pedroso e Silva de Azevedo,
- Vania Aparecida Vicente,
- Sybren de Hoog,
- Sybren de Hoog,
- Sybren de Hoog,
- Sybren de Hoog
Affiliations
- Gheniffer Fornari
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Renata Rodrigues Gomes
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Juliana Degenhardt-Goldbach
- Embrapa Forestry, Brazilian Agricultural Research Corporation (EMBRAPA), Colombo, Brazil
- Suelen Silvana dos Santos
- Department of Clinical and Pharmacological Analysis, College of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil
- Sandro Rogério de Almeida
- Department of Clinical and Pharmacological Analysis, College of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil
- Germana Davila dos Santos
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Marisol Dominguez Muro
- Support and Diagnosis Unit, Mycology Laboratory, Hospital of Clinics, Federal University of Paraná, Curitiba, Brazil
- Cleusa Bona
- Department of Botany, Federal University of Paraná, Curitiba, Brazil
- Rosana Herminia Scola
- Hospital of Clinics, Federal University of Paraná, Curitiba, Brazil
- Edvaldo S. Trindade
- Department of Cell Biology, Federal University of Paraná, Curitiba, Brazil
- Israel Henrique Bini
- Department of Cell Biology, Federal University of Paraná, Curitiba, Brazil
- Lisandra Santos Ferreira-Maba
- Department of Cell Biology, Federal University of Paraná, Curitiba, Brazil
- Daiane Rigoni Kestring
- Embrapa Forestry, Brazilian Agricultural Research Corporation (EMBRAPA), Colombo, Brazil
- Mariana Machado Fidelis do Nascimento
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Bruna Jacomel Favoreto de Souza Lima
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Morgana F. Voidaleski
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Douglas André Steinmacher
- Vivetech Agrociências, Paraná, Brazil
- Bruna da Silva Soley
- Department of Pharmacology, Federal University of Paraná, Curitiba, Brazil
- Shuwen Deng
- 0Department of Medical Microbiology, People’s Hospital of Suzhou National New & Hi-Tech Industrial Development Zone, Jiangsu, China
- Anamelia Lorenzetti Bocca
- 1Dermato-Immunology Laboratory, Institute of Biological Sciences, Federal University of Para, Marituba, Brazil
- Moises B. da Silva
- 2Department of Cell Biology, University of Brasília (UnB), Brasília, Brazil, 13 Department of Medicine, Federal University of Maranhão, São Luís, Brazil
- Claudio G. Salgado
- 1Dermato-Immunology Laboratory, Institute of Biological Sciences, Federal University of Para, Marituba, Brazil
- Conceição Maria Pedroso e Silva de Azevedo
- 3Department of Medicine, Federal University of Maranho, São Lus, Brazil
- Vania Aparecida Vicente
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Sybren de Hoog
- Microbiology, Parasitology and Pathology Post-graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Sybren de Hoog
- 0Department of Medical Microbiology, People’s Hospital of Suzhou National New & Hi-Tech Industrial Development Zone, Jiangsu, China
- Sybren de Hoog
- 4Westerdijk Fungal Biodiversity Institute, Utrecht, Netherlands
- Sybren de Hoog
- 5Center of Expertise in Mycology Radboudumc/CWZ, Nijmegen, Netherlands
- DOI
- https://doi.org/10.3389/fmicb.2018.02211
- Journal volume & issue
-
Vol. 9
Abstract
The fungal genus Fonsecaea comprises etiological agents of human chromoblastomycosis, a chronic implantation skin disease. The current hypothesis is that patients acquire the infection through an injury from plant material. The present study aimed to evaluate a model of infection in plant and animal hosts to understand the parameters of trans-kingdom pathogenicity. Clinical strains of causative agents of chromoblastomycosis (Fonsecaea pedrosoi and Fonsecaea monophora) were compared with a strain of Fonsecaea erecta isolated from a living plant. The clinical strains of F. monophora and F. pedrosoi remained concentrated near the epidermis, whereas F. erecta colonized deeper plant tissues, resembling an endophytic behavior. In an invertebrate infection model with larvae of a beetle, Tenebrio molitor, F. erecta exhibited the lowest survival rates. However, F. pedrosoi produced dark, spherical to ovoidal cells that resembled muriform cells, the invasive form of human chromoblastomycosis confirming the role of muriform cells as a pathogenic adaptation in animal tissues. An immunologic assay in BALB/c mice demonstrated the high virulence of saprobic species in animal models was subsequently controlled via host higher immune response.
Keywords
