A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice

Qin Peng; Deng Luchang; Chen Weilan; Huang Juan; Fan Shijun; Tu Bin; Tan Jun; Yuan Hua; Wang Yuping; Ma Bingtian; Li Shigui

Rice Science (Sep 2020)

A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice

Qin Peng,
Deng Luchang,
Chen Weilan,
Huang Juan,
Fan Shijun,
Tu Bin,
Tan Jun,
Yuan Hua,
Wang Yuping,
Ma Bingtian,
Li Shigui

Affiliations

Qin Peng: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Deng Luchang: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China; Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Chen Weilan: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Huang Juan: Characteristic Crop Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China
Fan Shijun: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Tu Bin: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Tan Jun: Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Yuan Hua: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Wang Yuping: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Ma Bingtian: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Li Shigui: Rice Research Institute/State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China; Corresponding author.

Journal volume & issue: Vol. 27, no. 5
pp. 396 – 404

Abstract

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Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant (ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.

Published in Rice Science

ISSN: 1672-6308 (Print); 1876-4762 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Plant culture
Website: https://www.journals.elsevier.com/rice-science

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