First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

Daniel Goldenberger; Danielle Vuichard-Gysin; Kathrin Herzog; Vladimira Hinić; Helena Seth-Smith; Adrian Egli

IDCases (Jan 2020)

First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

Daniel Goldenberger,
Danielle Vuichard-Gysin,
Kathrin Herzog,
Vladimira Hinić,
Helena Seth-Smith,
Adrian Egli

Affiliations

Daniel Goldenberger: Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland; Corresponding author at: Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Petergraben 4, CH-4031, Basel, Switzerland.
Danielle Vuichard-Gysin: Division of Infectious Diseases and Hospital Epidemiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland
Kathrin Herzog: Laboratory for Clinical Microbiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland
Vladimira Hinić: Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland
Helena Seth-Smith: Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Switzerland
Adrian Egli: Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Switzerland

Journal volume & issue: Vol. 22
p. e00933

Abstract

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We report on the first case of multiresistant Pseudomonas aeruginosa harbouring the metallo-β-lactamase IMP-15 isolated in Switzerland from a patient repatriated from Cambodia. The laboratory diagnosis of IMP-15 was hampered by two negative tests for carbapenemase detection. The carbapenemase gene was subsequently detected by whole genome sequencing and the isolate further characterised by various phenotypic and genotypic analyses.

Published in IDCases

ISSN: 2214-2509 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: http://www.journals.elsevier.com/idcases/

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