Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

Dejia Zhang; Yu Qi; Yaxuan Cui; Weiyi Song; Xinrui Wang; Mingyuan Liu; Xuepeng Cai; Xuenong Luo; Xiaolei Liu; Shumin Sun; Shumin Sun

doi:10.3389/fcimb.2021.762472

Frontiers in Cellular and Infection Microbiology (Nov 2021)

Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

Dejia Zhang,
Yu Qi,
Yaxuan Cui,
Weiyi Song,
Xinrui Wang,
Mingyuan Liu,
Xuepeng Cai,
Xuenong Luo,
Xiaolei Liu,
Shumin Sun,
Shumin Sun

Affiliations

Dejia Zhang: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Yu Qi: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Yaxuan Cui: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Weiyi Song: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Xinrui Wang: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Mingyuan Liu: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, China
Xuepeng Cai: Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etioloical Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricuitural Sciences, Lanzhou, China
Xuenong Luo: Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etioloical Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricuitural Sciences, Lanzhou, China
Xiaolei Liu: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, China
Shumin Sun: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China
Shumin Sun: College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China

DOI: https://doi.org/10.3389/fcimb.2021.762472
Journal volume & issue: Vol. 11

Abstract

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Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

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