Jichu yixue yu linchuang (Mar 2020)

Estrogen promotes proliferation and invasion of astrocyte cell line U-87

  • LIU Yan-min, FEI Yi-lin, LI Xing-miao, WANG Ning, CHEN Mo, JIANG Xiu-xiu

Journal volume & issue
Vol. 40, no. 3
pp. 305 – 309

Abstract

Read online

Objective To explore the relationships between the estrogen and astrocyte cell proliferation as well as invasion. Methods Astrocyte cell line U-87 was cultured in vitro and treated with 0, 10－12, 10－10, 10－8 and 10－6 mol/L estrogen. The gene and protein expressions were analyzed by qPCR, Western blot, ELISA; and the cell proliferation and invasion was analyzed by CCK-8 kits and transwell experiments. Results Estrogen significantly inhibited IL-1, TNF-α, IL-6 expression and promoted the expression of NGF and PGE2 (P＜0.05). Furthermore, estrogen inhibited aquaporin 2(AQP2) expression (P＜0.05). The proliferation and invasion experiments showed that estrogen promoted the U-87 cell proliferation and invasion (P＜0.05). Conclusions Estrogen down-regulates the expression of inflammatory factors, inhibites the expression of AQP2, promotes the proliferation and invasion of U-87 cells.

Keywords