Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology

Abstract

An amperometric biosensor based on the bioelectrocatalytic measurement principle for the detection of adrenaline has been developed. The adrenaline sensor has been prepared by modification of a platinum thin-film electrode with a pyrroloquinoline quinone-dependent glucose dehydrogenase. The enzyme was immobilized via cross-linking method. Lower detection limit of 1 nM of adrenaline has been achieved by measuring at physiological level at pH 7.4.

Keywords

• chip-based biosensor
• adrenaline
• glucose dehydrogenase
• bioelectrocatalysis