Morphological Diversity of Chrysanthemum (Dendranthema grandiflora Tzvelev) Genotypes induced by EMS (Ethyl methane sulfonate)

Abstract

Ethyl methane sulfonate (EMS) is one of chemical mutagen which it known to cause point mutations in plants. The mutation was changing in one or some character based on Basa nucleotide. The changing shown by morphological characteristic to create novelties and superior characters. This study aim was to evaluate nine chrysanthemum genotypes of the MV4 generation by EMS treatment. The observation parameters were quantitative and qualitative characters. The quantitative charactera are number of flower buds, flower diameter (cm), flower stalk length (cm), leaf length (cm), leaf width (cm), plant height (cm), and stem diameter (cm). And the qualitative ones are stem, leaf, and ribbon flower colour. Morphological character analysis used the Statistical Tools for Agricultural Research (STAR) statistical program. One-way ANOVA was used to compare the means of different genotypes, and means were separated using Duncan’s Multiple Range Test (DMRT) at a significance level of 0.05. The highest plant, stem diameter, and flower stalk length were obtained genotype JE0 as a contol plant. Genotype JE4.8 as a selected plant showed the diameter of flower buds, number of flower buds, leaf length, leaf width and stem diameter. JE2.44 (Purple group 75 A) and JE5.31 (Red purple group 60 A) were significantly different of colour JE0 as control (Red purple group 70 B).

Keywords

• chrysanthemum
• genotype
• morphological character
• mutation