Complete chloroplast genome of Caragana kozlowii, an alpine shrub endemic to China

Abstract

Endemic to the southeast boundary of Tibet plateau, the medicinal alpine shrub Caragana kozlowii is a threatened species. The genetic and molecular data about it is insufficient. In this paper, the complete chloroplast (cp) genome sequence of C. kozlowii was sequenced by the Illumina sequencing platform. The result suggests that C. kozlowii cp genome is relatively short because it lacks an Inverted Repeat (IR) region. It was 131,274 bp in length, containing one large and one small single copy region (SSC and LSC) of 85,281 and 18,919 bp separated by an inverted repeat region (IR) of 27,074 bp, respectively. It contains unique 110 genes, including 76 protein-coding genes, 30 tRNAs, and 4 rRNAs. Similar to the other Inverted Repeat-Lacking Clade (IRLC) plastomes, rpl22 and rps16 are absent. The cp genome has 34.5% GC content. Phylogenetic analysis based on ten chloroplast genomes indicates that C. kozlowii is closely related to C. rosea.

Keywords

• caragana
• chloroplast genome
• phylogenetic analysis