Microbiology Spectrum (Nov 2024)

First isolation of Campylobacter vicugnae sp. nov. in humans suffering from gastroenteritis

  • Quentin Jehanne,
  • Lucie Bénéjat,
  • Lamia Azzi Martin,
  • Victoria Korolik,
  • Astrid Ducournau,
  • Johanna Aptel,
  • Armelle Ménard,
  • Marine Jauvain,
  • Christophe Aguilera,
  • Alice Doreille,
  • Laurent Mesnard,
  • Catherine Eckert,
  • Philippe Lehours

DOI
https://doi.org/10.1128/spectrum.01523-24
Journal volume & issue
Vol. 12, no. 11

Abstract

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ABSTRACT The present study describes the first isolation of a recently described Campylobacter species, Campylobacter vicugnae, in humans. The isolates were recovered by two independent French laboratories in 2020 and 2022 from a man and a woman suffering from gastroenteritis. Biochemical and growth characteristics, and electron microscopy for these two strains indicated that they belong to Campylobacter genus. 16S rDNA and GyrA-based phylogeny, as well as average nucleotide identity and in silico DNA-DNA Hybridization analyses revealed that both strains belong to the Campylobacter vicugnae species. Both isolates possess a complete cytolethal distending toxin (CDT) locus with cdtA, cdtB, and cdtC, and features of CDT activity were demonstrated in vitro with Caco-2 intestinal epithelial cells. Our data suggest that these two isolates of C. vicugnae were associated with gastroenteritis in humans and induced major cytopathogenic effects in vitro. C. vicugnae is likely to be a novel human pathogen, with a source of foodborne infection that needs to be determined.IMPORTANCECampylobacter species that display toxicity features are a worldwide public health issue. In clinical contexts, it is crucial to identify which isolate could be an urgent threat to a patient. Actual and widely used laboratory methods such as mass spectrometry or PCR may be flawed in the field of species identification. In contrast, the present study shows that next-generation sequencing allows to precisely identify isolates to species level that may have been omitted otherwise. Moreover, it helps to identify emerging species before they become a threat to human health. Recovery of a new Campylobacter species in human sample, such as the new species “Campylobacter vicugnae,” is an important step for the identification of emerging pathogens posing threat to global health.

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