Frontiers in Immunology (Apr 2022)

CD45RB Glycosylation and Ig Isotype Define Maturation of Functionally Distinct B Cell Subsets in Human Peripheral Blood

  • Jana Koers,
  • Sabrina Pollastro,
  • Simon Tol,
  • Ingrid Pico-Knijnenburg,
  • Ninotska I. L. Derksen,
  • Pauline A. van Schouwenburg,
  • Mirjam van der Burg,
  • S. Marieke van Ham,
  • S. Marieke van Ham,
  • Theo Rispens

DOI
https://doi.org/10.3389/fimmu.2022.891316
Journal volume & issue
Vol. 13

Abstract

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Glycosylation of CD45RB (RB+) has recently been identified to mark antigen-experienced B cells, independent of their CD27 expression. By using a novel combination of markers including CD45RB glycosylation, CD27 and IgM/IgD isotype expression we segregated human peripheral blood B cell subsets and investigated their IGHV repertoire and in vitro functionality. We observed distinct maturation stages for CD27-RB+ cells, defined by differential expression of non-switched Ig isotypes. CD27-RB+ cells, which only express IgM, were more matured in terms of Ig gene mutation levels and function as compared to CD27-RB+ cells that express both IgM and IgD or cells that were CD27-RB-. Moreover, CD27-RB+IgM+ cells already showed remarkable rigidity in IgM isotype commitment, different from CD27-RB+IgMD+ and CD27-RB- cells that still demonstrated great plasticity in B cell fate decision. Thus, glycosylation of CD45RB is indicative for antigen-primed B cells, which are, dependent on the Ig isotype, functionally distinct.

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