PeposX-Exhaust: A lightweight and efficient tool for identification of short peptides

Wanshun Liu; Mouming Zhao; Lishe Gan; Baoguo Sun; Shiqi He; Yang Liu; Lei Liu; Wu Li; Jing Chen; Yang Liu; Jianan Zhang; Jucai Xu

Food Chemistry: X (Jun 2024)

PeposX-Exhaust: A lightweight and efficient tool for identification of short peptides

Wanshun Liu,
Mouming Zhao,
Lishe Gan,
Baoguo Sun,
Shiqi He,
Yang Liu,
Lei Liu,
Wu Li,
Jing Chen,
Yang Liu,
Jianan Zhang,
Jucai Xu

Affiliations

Wanshun Liu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Mouming Zhao: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Lishe Gan: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
Baoguo Sun: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China
Shiqi He: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Yang Liu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China; College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
Lei Liu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Wu Li: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Jing Chen: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Yang Liu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China
Jianan Zhang: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Corresponding authors at: Room 323, Building 13 of SCUT, No.381 Wushan Rd., Tianhe District, Guangzhou 510640, China(Jianan Zhang).
Jucai Xu: Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China; Corresponding authors at: Room 323, Building 13 of SCUT, No.381 Wushan Rd., Tianhe District, Guangzhou 510640, China(Jianan Zhang).

Journal volume & issue: Vol. 22
p. 101249

Abstract

Read online

Short peptides have become the focus of recent research due to their variable bioactivities, good digestibility and wide existences in food-derived protein hydrolysates. However, due to the high complexity of the samples, identifying short peptides still remains a challenge. In this work, a tool, named PeposX-Exhaust, was developed for short peptide identification. Through validation with known peptides, PeposX-Exhaust identified all the submitted spectra and the accuracy rate reached 75.36%, and the adjusted accuracy rate further reached 98.55% when with top 5 candidates considered. Compared with other tools, the accuracy rate by PeposX-Exhaust was at least 70% higher than two database-search tools and 15% higher than the other two de novo-sequencing tools, respectively. For further application, the numbers of short peptides identified from soybean, walnut, collagen and bonito protein hydrolysates reached 1145, 628, 746 and 681, respectively. This fully demonstrated the superiority of the tool in short peptide identification.

Published in Food Chemistry: X

ISSN: 2590-1575 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply; Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-x

About the journal

Abstract

Keywords