Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field

Jianghong Huang; Daming Wang; Jielin Chen; Wei Liu; Li Duan; Wei You; Weimin Zhu; Jianyi Xiong; Daping Wang

Saudi Pharmaceutical Journal (May 2017)

Osteogenic differentiation of bone marrow mesenchymal stem cells by magnetic nanoparticle composite scaffolds under a pulsed electromagnetic field

Jianghong Huang,
Daming Wang,
Jielin Chen,
Wei Liu,
Li Duan,
Wei You,
Weimin Zhu,
Jianyi Xiong,
Daping Wang

Affiliations

Jianghong Huang: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Daming Wang: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Software Town of Shenzhen Universiade, Shenzhen Longer3d Technology, Shenzhen 518116, China
Jielin Chen: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Wei Liu: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Li Duan: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Wei You: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Weimin Zhu: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Jianyi Xiong: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China
Daping Wang: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Shenzhen Laboratory of Digital Orthopedic Engineering, Shenzhen Second People’s Hospital, Shenzhen 518035, China; Corresponding author at: Shenzhen National Key Department of Orthopedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China.

Journal volume & issue: Vol. 25, no. 4
pp. 575 – 579

Abstract

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This study was conducted to investigate the effect of magnetic nanoparticle composite scaffold under a pulsed electromagnetic field on bone marrow mesenchymal stem cells (BMSCs), which was achieved by examining the biological behaviors of cell adhesion, proliferation and differentiation on the surface of the scaffolds. This may provide some experimental evidence for the use of magnetic nanoparticles in medical application. The magnetic nanoparticle composite scaffolds were evaluated and characterized by the following indexes: the cell proliferation was detected by the CCK-8 method, the alkaline phosphatase (ALP) activity was examined by a detection kit, and the expression of type I collagen and osteocalcin gene were evaluated by RT-PCR. The CCK-8 test showed that there was no significant difference in Group A (BMSCs-seeded magnetic scaffolds under the electromagnetic field), B (BMSCs-seeded magnetic scaffolds) and C (BMSCs cultured alone) (P > 0.05). The value for the ALP activity in Group A was higher than the other two groups. In addition, the RT-PCR results showed that the expression of type I collagen gene in Group A was enhanced (P 0.05). To conclude, magnetic nanoparticles may induce the osteogenic differentiation with the action of the pulsed electromagnetic field. Keywords: Magnetic nanoparticles, Bone marrow mesenchymal stem cells, Osteogenic differentiation, Pulsed electromagnetic field

Published in Saudi Pharmaceutical Journal

ISSN: 1319-0164 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/saudi-pharmaceutical-journal/

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