Antibiotic resistance in a predominantly occurring Gram-negative bacterial community from treated sewage to assess the need for going beyond coliform standards

Abstract

Antibiotic resistance surveillance is an objective of global action plan on antimicrobial resistance endorsed by the World Health Organization. The current study reports the identification of frequently occurring Gram-negative bacterial community (GNBC) previously isolated from municipal treated wastewater and their antibiotic resistance profiles. Further, the genes responsible for extended-spectrum beta lactamases (ESBL) activity were identified in ESBL-positive organisms. The isolates were characterized using biochemical assays and identification was confirmed by VITEK®2 automated system. Antibiotic susceptibility testing against seven different classes of antibiotics was also performed on the same system using AST-N280 cards. The most dominant isolates identified were Acinetobacter baumannii, Morganella morganii, Kluyvera intermedia, Stenotrophomonas maltophilia, Escherichia coli, Aeromonas hydrophila/caviae, Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter freundii. The isolates were observed to be significantly resistant against the antibiotics amoxicillin-clavulanic acid, cefuroxime, cefuroxime axetil and colistin. Two of the isolates, E. cloacae sp. dissolvens and S. maltophilia, were found to be positive for ESBL activity encoded by blaCTX-M gene. The possible intrusion of hospital wastewater in domestic sewage is also discussed. This study may help assess the risk of wastewater reuse by detecting dominant bacteria as a step towards the development of new microbiological standards. HIGHLIGHTS Stenotrophomonas maltophilia found to be most resistant and E. coli least resistant among the isolates.; Five isolates found to be resistant to the last resort antibiotic Colistin.; Molecular determinant of two extended spectrum beta lactamase producing isolates found to be blaCTX-M gene.; This report might aid in devising a suitable strategy for reducing risk to human health upon treated wastewater reuse.;

Keywords

• esbl
• gram-negative bacterial community
• multiple drug resistance
• treated municipal sewage
• vitek®2