Acta Cirúrgica Brasileira (Aug 2014)

Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

  • André Roberto Bussmann,
  • Marcos Antônio Marton Filho,
  • Marília Pinheiro Módolo,
  • Renata Pinheiro Módolo,
  • Patrícia Amado,
  • Maria Aparecida Custódio Domingues,
  • Yara Marcondes Machado Castiglia,
  • Norma Sueli Pinheiro Módolo

DOI
https://doi.org/10.1590/S0102-86502014000800006
Journal volume & issue
Vol. 29, no. 8
pp. 515 – 521

Abstract

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PURPOSE:To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM 1 and IL 18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury.METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg·kg1·d 1; and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg·kg 1·d1. The NGALp, NGALu, KIM 1, IL 18 and creatinine levels and the kidney histology were analysed. The significance level was established as p S ≈ CA. The NGALp, NGALu and IL 18 levels exhibited similar behaviour in all the groups. KIM 1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA.CONCLUSION:Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury.

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