Properties and anticancer effects of plasma-activated medium stored at different temperatures

Runze Fan; Xinyi Zhao; Miao Qi; Huaiyan Zhang; Xinying Zhang; Jin Zhang; Qiaosong Li; Dehui Xu

doi:10.1063/5.0120681

AIP Advances (Sep 2022)

Properties and anticancer effects of plasma-activated medium stored at different temperatures

Runze Fan,
Xinyi Zhao,
Miao Qi,
Huaiyan Zhang,
Xinying Zhang,
Jin Zhang,
Qiaosong Li,
Dehui Xu

Affiliations

Runze Fan: State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China
Xinyi Zhao: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Miao Qi: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Huaiyan Zhang: School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Xinying Zhang: State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China
Jin Zhang: State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China
Qiaosong Li: State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China
Dehui Xu: State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, China

DOI: https://doi.org/10.1063/5.0120681
Journal volume & issue: Vol. 12, no. 9
pp. 095022 – 095022-7

Abstract

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Plasma-activated solution (PAS) has attracted wide attention in cancer treatment because it can treat deep tumors and offer storability. The changes in reactive species and physicochemical properties of PAS during storage can affect its anticancer effect. In this study, the plasma-activated medium (PAM) was prepared by treating RPMI 1640 medium with afterglow gas generated by a custom-built air surface dielectric barrier discharge device. PAM was stored at four common temperature conditions (25 °C, 4 °C, −20 °C, and −80 °C) for 1 day, 4 days, and 7 days, and then, the physicochemical properties, reactive oxygen and nitrogen species (RONS), and the anti-cancer effect on A549 cells under different storage conditions were compared. The results showed that PAM exhibited different anticancer effects at different storage temperatures over a 7 day storage period. The anticancer ability of PAM under 80 °C storage remained stable after 7 days of storage and decreased at all other temperatures. These results were also verified by apoptosis results, and the differences were mainly related to the concentration of H2O2 and NO2−, and oxido reduction potential. Our results provided a theoretical basis for the storage of PAM and its application in anticancer therapy.

Published in AIP Advances

ISSN: 2158-3226 (Online)
Publisher: AIP Publishing LLC
Country of publisher: United States
LCC subjects: Science: Physics
Website: http://aipadvances.aip.org/

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