Nature Communications (Jan 2023)
Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling
- Julia Ast,
- Daniela Nasteska,
- Nicholas H. F. Fine,
- Daniel J. Nieves,
- Zsombor Koszegi,
- Yann Lanoiselée,
- Federica Cuozzo,
- Katrina Viloria,
- Andrea Bacon,
- Nguyet T. Luu,
- Philip N. Newsome,
- Davide Calebiro,
- Dylan M. Owen,
- Johannes Broichhagen,
- David J. Hodson
Affiliations
- Julia Ast
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Daniela Nasteska
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Nicholas H. F. Fine
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Daniel J. Nieves
- Institute for Immunology and Immunotherapy, and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Zsombor Koszegi
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Yann Lanoiselée
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Federica Cuozzo
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Katrina Viloria
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Andrea Bacon
- Genome Editing Facility, Technology Hub, University of Birmingham
- Nguyet T. Luu
- National Institute for Health Research Biomedical Research Centre at University Hospitals Birmingham NHS Foundation Trust, University of Birmingham
- Philip N. Newsome
- National Institute for Health Research Biomedical Research Centre at University Hospitals Birmingham NHS Foundation Trust, University of Birmingham
- Davide Calebiro
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Dylan M. Owen
- Institute for Immunology and Immunotherapy, and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Johannes Broichhagen
- Leibniz-Forschungsinstitut für Molekulare Pharmakologie
- David J. Hodson
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- DOI
- https://doi.org/10.1038/s41467-022-35716-1
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 15
Abstract
Visualizing endogenous GPCRs is challenging. Here the authors generate mice with an enzyme self-label genome-edited into the endogenous glucagon-like peptide-1 receptor locus, design fluorescent dyes for specific labelling in complex tissue, and reveal tissue-level organisation and dynamics of an endogenous class B GPCR.
