Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli

Mengxun Shi; Mengxun Shi; Mengdi Li; Anran Yang; Xue Miao; Liu Yang; Jagroop Pandhal; Huibin Zou; Huibin Zou

doi:10.3389/fbioe.2022.919969

Frontiers in Bioengineering and Biotechnology (Jun 2022)

Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli

Mengxun Shi,
Mengxun Shi,
Mengdi Li,
Anran Yang,
Xue Miao,
Liu Yang,
Jagroop Pandhal,
Huibin Zou,
Huibin Zou

Affiliations

Mengxun Shi: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Mengxun Shi: Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom
Mengdi Li: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Anran Yang: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Xue Miao: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Liu Yang: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Jagroop Pandhal: Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom
Huibin Zou: State Key Laboratory Base of Eco-Chemical Engineering, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China
Huibin Zou: CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China

DOI: https://doi.org/10.3389/fbioe.2022.919969
Journal volume & issue: Vol. 10

Abstract

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Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1Ps6-19) was commonly utilized in the de novo biosynthesis of PLA from biomass. This study tested alternative class I PHA synthase (PhaCCs) from Chromobacterium sp. USM2 in engineered Escherichia coli for the de novo biosynthesis of PLA from glucose. The results indicated that PhaCCs had better performance in PLA production than that of class II synthase PhaC1Ps6-19. In addition, the sulA gene was engineered in PLA-producing strains for morphological engineering. The morphologically engineered strains present increased PLA production. This study also tested fused propionyl-CoA transferase and lactate dehydrogenase A (fused PctCp/LdhA) in engineered E. coli and found that fused PctCp/LdhA did not apparently improve the PLA production. After systematic engineering, the highest PLA production was achieved by E. coli MS6 (with PhaCCs and sulA), which could produce up to 955.0 mg/L of PLA in fed-batch fermentation with the cell dry weights of 2.23%, and the average molecular weight of produced PLA could reach 21,000 Da.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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