The cytosolic isoform of triosephosphate isomerase, ZmTPI4, is required for kernel development and starch synthesis in maize (Zea mays L.)

Wenyu Li; Han Wang; Qiuyue Xu; Long Zhang; Yan Wang; Yongbiao Yu; Xiangkun Guo; Zhiwei Zhang; Yongbin Dong; Yuling Li

Crop Journal (Apr 2024)

The cytosolic isoform of triosephosphate isomerase, ZmTPI4, is required for kernel development and starch synthesis in maize (Zea mays L.)

Wenyu Li,
Han Wang,
Qiuyue Xu,
Long Zhang,
Yan Wang,
Yongbiao Yu,
Xiangkun Guo,
Zhiwei Zhang,
Yongbin Dong,
Yuling Li

Affiliations

Wenyu Li: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Han Wang: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Qiuyue Xu: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Long Zhang: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Yan Wang: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Yongbiao Yu: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Xiangkun Guo: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Zhiwei Zhang: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Yongbin Dong: State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China
Yuling Li: Corresponding author.; State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Longzi Lake Campus, Zhengzhou 450046, Henan, China

Journal volume & issue: Vol. 12, no. 2
pp. 401 – 410

Abstract

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Triosephosphate isomerase (TPI) is an enzyme that functions in plant energy production, accumulation, and conversion. To understand its function in maize, we characterized a maize TPI mutant, zmtpi4. In comparison to the wild type, zmtpi4 mutants showed altered ear development, reduced kernel weight and starch content, modified starch granule morphology, and altered amylose and amylopectin content. Protein, ATP, and pyruvate contents were reduced, indicating ZmTPI4 was involved in glycolysis. Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI, the zmtpi4 mutant showed reduced leaf size and chlorophyll content. Overexpression of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight, suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content. We conclude that ZmTPI4 functions in maize kernel development, starch synthesis, glycolysis, and photosynthesis.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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