Drug Design, Development and Therapy (Nov 2019)
Effects Of Triptolide On Tooth Movement And Root Resorption In Rats
Abstract
Fan Yang,1,2 Xu Xia Wang,1,3 Dan Ma,1,2 Qun Cui,1,2 De Hua Zheng,1,2 Xiao Can Liu,1,2 Jun Zhang1,2 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of ChinaCorrespondence: Jun ZhangDepartment of Orthodontics, School of Stomatology, Shandong University, 44 Wenhua West Road, Jinan 250012, People’s Republic of ChinaTel +86 139 5310 9816Fax +86-531-88565657Email [email protected]: The aim of this study was to investigate the effects of triptolide on the tooth movement and root resorption in rats during orthodontic treatment.Material and methods: A total of 48 male Wistar rats were divided into three groups of 16 each. The right maxillary first molars of rats were drawn mesially by closed coil nickel–titanium spring with a force of 50 g. The two experimental groups received intraperitoneal injections of triptolide for 14 days at a dose of 15 μg/kg/day and 30 μg/kg/day, respectively. The control group received vehicle injections. After 14 days, the rats were humanely killed. The amount of tooth movement was measured. Eight rats from each group were randomly chosen for analysis of the percentage of root resorption area by scanning electron microscopy. For the remaining eight rats in each group, the H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis were performed.Results: The amount of tooth movement and the ratio of root resorption area were significantly decreased in the triptolide-treated rats. The number of TRAP-positive cells was significantly lower in triptolide-treated groups. Moreover, the expression of nuclear factor kappa B ligand (RANKL) was reduced. In contrast, the expression of osteoprotegerin was significantly up-regulated. In the tension side, the expressions of runt-related transcription factor 2 and osteocalcin were significantly enhanced by triptolide injection.Conclusion: Triptolide injection could arrest orthodontic tooth movement and reduce root resorption in rats via inhibition of osteoclastogenesis. In addition, triptolide may exert a positive effect on osteoblastogenesis.Keywords: triptolide, osteoclast, scanning electron microscope, tooth movement, bone formation
