Starch granules in algal cells play an inherent role to shape the popular SSC signal in flow cytometry

Abstract

Abstract Objective Flow cytometry (FC) is probably the most important technique for single-cell analysis. It’s precisely, rapid, and suitable for multidimensional single-cell analysis. The commonly used side scatter (SSC) intensity determined by FC is often interpreted as a measure of the internal cellular complexity of cells. In simple terms, the more structured a cell is, the higher the SSC intensity quantified by FC. Nevertheless, most of the studies that support this interpretation are based on data derived from animal or human cell lines and while it is assumed that the results can also be transferred to plant or algal cell lines, the details remain unclear. The objective of the recent work is to clarify the interpretation of the SSC signal from algal cells. Results Algal lipid droplets and their starch play an inherent role to shape the popular SSC signal derived from FC. This was shown by a theoretical approach based on Lorenz–Mie theory. These results were supported by experiments with different model cultures of Chlamydomonas reinhardtii in which a high linear correlation was observed between the SSC signal and the ‘physical’ starch quantity.

Keywords

• Diurnal rhythm
• single-cell analysis
• flow cytometry
• starch
• Lorenz–Mie theory; light scattering