Infection and Drug Resistance (Mar 2023)

Molecular Epidemiology of Clinical Mycobacterium tuberculosis Isolates from Southern Xinjiang, China Using Spoligotyping and 15-Locus MIRU-VNTR Typing

  • Yin C,
  • Mijiti X,
  • Liu H,
  • Wang Q,
  • Cao B,
  • Anwaierjiang A,
  • Li M,
  • Liu M,
  • Jiang Y,
  • Xu M,
  • Wan K,
  • Zhao X,
  • Li G,
  • Xiao H

Journal volume & issue
Vol. Volume 16
pp. 1313 – 1326

Abstract

Read online

Chunjie Yin,1,* Xiaokaiti Mijiti,2,* Haican Liu,3,* Quan Wang,2 Bin Cao,3,4 Aiketaguli Anwaierjiang,5 Machao Li,3 Mengwen Liu,1 Yi Jiang,3 Miao Xu,2 Kanglin Wan,3 Xiuqin Zhao,3 Guilian Li,3 Hui Xiao1 1School of Public Health, Xinjiang Medical University, Urumqi, People’s Republic of China; 2The Eighth Affiliated Hospital of Xinjiang Medical University, Urumqi, People’s Republic of China; 3State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China; 4School of Public Health, University of South China, Hengyang, People’s Republic of China; 5College of Xinjiang Uyghur Medicine, Hetian, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guilian Li; Hui Xiao, Email [email protected]; [email protected]: In the last decades, the molecular epidemiological investigation of Mycobacterium tuberculosis has significantly increased our understanding of tuberculosis epidemiology. However, few such studies have been done in southern Xinjiang, China. We aimed to clarify the molecular epidemic characteristics and their association with drug resistance in the M. tuberculosis isolates circulating in this area.Methods: A total of 347 isolates obtained from southern Xinjiang, China between Sep, 2017 and Sep, 2019 were included to characterize using a 15-locus MIRU-VNTR (VNTR-15China) typing and spoligotyping, and test for drug susceptibility profiles. Then the lineages and clustering of the isolates were analyzed, as well as their association with drug resistance.Results: Spoligotyping results showed that 60 spoligotype international types (SITs) containing 35 predefined SITs and 25 Orphan or New patterns, and 12 definite genotypes were found, and the top three prevalent genotypes were Beijing genotype (207, 59.7%), followed by CAS1-Delhi (46, 13.6%), and Ural-2 (30, 8.6%). The prevalence of Beijing genotype infection in the younger age group (≤ 30) was more frequent than the two older groups (30~59 and ≥ 60 years old, both P values < 0.05). The Beijing genotype showed significantly higher prevalence of resistance to isoniazid, rifampicin, ethambutol, multi-drug or at least one drug than the non-Beijing genotype (All P values ≤ 0.05). The estimated proportion of tuberculosis cases due to transmission was 18.4% according to the cluster rate acquired by VNTR-15China typing, and the Beijing genotype was the risk factor for the clustering (OR 9.15, 95% CI: 4.18– 20.05).Conclusion: Our data demonstrated that the Beijing genotype is the dominant lineage, associated with drug resistance, and was more likely to infect young people and contributed to tuberculosis transmission in southern Xinjiang, China. These findings will contribute to a better understanding of tuberculosis epidemiology in this area.Keywords: MIRU-VNTR, spoligotyping, Beijing genotype, drug resistance, genetic diversity, Mycobacterium tuberculosis

Keywords