Frontiers in Microbiology (Oct 2017)
Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis
- Vania A. Vicente,
- Vania A. Vicente,
- Vinícius A. Weiss,
- Vinícius A. Weiss,
- Amanda Bombassaro,
- Leandro F. Moreno,
- Leandro F. Moreno,
- Leandro F. Moreno,
- Flávia F. Costa,
- Roberto T. Raittz,
- Aniele C. Leão,
- Aniele C. Leão,
- Aniele C. Leão,
- Renata R. Gomes,
- Anamelia L. Bocca,
- Gheniffer Fornari,
- Raffael J. A. de Castro,
- Jiufeng Sun,
- Helisson Faoro,
- Michelle Z. Tadra-Sfeir,
- Valter Baura,
- Eduardo Balsanelli,
- Sandro R. Almeida,
- Suelen S. Dos Santos,
- Marcus de Melo Teixeira,
- Marcus de Melo Teixeira,
- Maria S. Soares Felipe,
- Mariana Machado Fidelis do Nascimento,
- Fabio O. Pedrosa,
- Maria B. Steffens,
- Maria B. Steffens,
- Derlene Attili-Angelis,
- Mohammad J. Najafzadeh,
- Flávio Queiroz-Telles,
- Flávio Queiroz-Telles,
- Emanuel M. Souza,
- Emanuel M. Souza,
- Sybren De Hoog,
- Sybren De Hoog,
- Sybren De Hoog
Affiliations
- Vania A. Vicente
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Vania A. Vicente
- Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil
- Vinícius A. Weiss
- Laboratory of Bioinformatics, Sector of Technological and Professional Education, Federal University of Paraná, Curitiba, Brazil
- Vinícius A. Weiss
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Amanda Bombassaro
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Leandro F. Moreno
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Leandro F. Moreno
- CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands
- Leandro F. Moreno
- Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands
- Flávia F. Costa
- Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil
- Roberto T. Raittz
- Laboratory of Bioinformatics, Sector of Technological and Professional Education, Federal University of Paraná, Curitiba, Brazil
- Aniele C. Leão
- Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil
- Aniele C. Leão
- Laboratory of Bioinformatics, Sector of Technological and Professional Education, Federal University of Paraná, Curitiba, Brazil
- Aniele C. Leão
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Renata R. Gomes
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Anamelia L. Bocca
- Department of Cell Biology, University of Brasília, Brasilia, Brazil
- Gheniffer Fornari
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Raffael J. A. de Castro
- Department of Cell Biology, University of Brasília, Brasilia, Brazil
- Jiufeng Sun
- Guangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China
- Helisson Faoro
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Michelle Z. Tadra-Sfeir
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Valter Baura
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Eduardo Balsanelli
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Sandro R. Almeida
- Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Sao Paulo, Brazil
- Suelen S. Dos Santos
- Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Sao Paulo, Brazil
- Marcus de Melo Teixeira
- Department of Cell Biology, University of Brasília, Brasilia, Brazil
- Marcus de Melo Teixeira
- 0Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States
- Maria S. Soares Felipe
- 1Department of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasilia, Brazil
- Mariana Machado Fidelis do Nascimento
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Fabio O. Pedrosa
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Maria B. Steffens
- Laboratory of Bioinformatics, Sector of Technological and Professional Education, Federal University of Paraná, Curitiba, Brazil
- Maria B. Steffens
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Derlene Attili-Angelis
- 2Division of Microbial Resources (DRM/CPQBA), University of Campinas, Campinas, Brazil
- Mohammad J. Najafzadeh
- 3Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
- Flávio Queiroz-Telles
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Flávio Queiroz-Telles
- 4Clinical Hospital of the Federal University of Paraná, Curitiba, Brazil
- Emanuel M. Souza
- Laboratory of Bioinformatics, Sector of Technological and Professional Education, Federal University of Paraná, Curitiba, Brazil
- Emanuel M. Souza
- Department of Biochemistry, Federal University of Paraná, Curitiba, Brazil
- Sybren De Hoog
- Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of Paraná, Curitiba, Brazil
- Sybren De Hoog
- CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands
- Sybren De Hoog
- Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands
- DOI
- https://doi.org/10.3389/fmicb.2017.01924
- Journal volume & issue
-
Vol. 8
Abstract
Fonsecaea and Cladophialophora are genera of black yeast-like fungi harboring agents of a mutilating implantation disease in humans, along with strictly environmental species. The current hypothesis suggests that those species reside in somewhat adverse microhabitats, and pathogenic siblings share virulence factors enabling survival in mammal tissue after coincidental inoculation driven by pathogenic adaptation. A comparative genomic analysis of environmental and pathogenic siblings of Fonsecaea and Cladophialophora was undertaken, including de novo assembly of F. erecta from plant material. The genome size of Fonsecaea species varied between 33.39 and 35.23 Mb, and the core genomes of those species comprises almost 70% of the genes. Expansions of protein domains such as glyoxalases and peptidases suggested ability for pathogenicity in clinical agents, while the use of nitrogen and degradation of phenolic compounds was enriched in environmental species. The similarity of carbohydrate-active vs. protein-degrading enzymes associated with the occurrence of virulence factors suggested a general tolerance to extreme conditions, which might explain the opportunistic tendency of Fonsecaea sibling species. Virulence was tested in the Galleria mellonella model and immunological assays were performed in order to support this hypothesis. Larvae infected by environmental F. erecta had a lower survival. Fungal macrophage murine co-culture showed that F. erecta induced high levels of TNF-α contributing to macrophage activation that could increase the ability to control intracellular fungal growth although hyphal death were not observed, suggesting a higher level of extremotolerance of environmental species.
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